Sirolimus Antikörper

Produktnummer ABIN1169577

Dieses Anti--Antikörper ist ein Schaf Polyklonal-Antikörper zur Detektion von in AP, ELISA und IA. Geeignet für Diverse Spezies.

Kurzübersicht für Sirolimus Antikörper (ABIN1169577)

Target: Sirolimus

Reaktivität: Diverse Spezies

Wirt: Schaf

Klonalität: Polyklonal

Applikation: Affinity Purification (AP), ELISA, Immunoassay (IA)

Produktdetails

Verwendungszweck: Sirolimus

Sensitivität: 10ng/mL
Prednisolone produces 59% inhibition in a competitive ELISA employing Prednisolone polyclonal Antibody

Aufreinigung: Salt fractionation.

Immunogen: Sirolimus-BTG

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application_Dilutions: 2.5 μg/mL

Tested_Applications: The antibody is suitable for the development of immunoassays or immunoaffinity purification columns.

Kommentare:

10 ng/mL Rapamycin (Sirolimus) produces 63 % inhibition in a competitive ELISA employing Sirolimus polyclonal antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 20 mM Phosphate, 150 mM Sodium Chloride. pH 7.4, 0.09 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Can be stored for up to 3 months at +2°C - +8°C. For long term storage, aliquot and store at ≤-20 °C. Avoid repeated freeze/thaw cycles. Product should be protected from light exposure.

Antigendetails

Target: Sirolimus

Substanzklasse: Chemical

Hintergrund: Dehydroepiandrosterone is the most abundant adrenal androgen in humans and exists predominately in a sulfated form (DHEAS) [1]. DHEA sulfotransferase, known as SULT2A1, converts the androgen precursor DHEA to its inactive sulfate ester, DHEAS. The unconjugated molecules can be converted directly to androgens. Dehydroepiandrosterone is converted to DHEAS in the adrenal glands and liver [2]. DHEA in adults is present at plasma concentrations 100 to 500 times higher than those of testosterone. DHEA can be metabolized to androgens and/or estrogens in the prostate [3]. Hepatic metabolites include 16alpha-hydroxy-DHEA, 7alpha-hydroxy-DHEA, and 7-oxo-DHEA [4]. 7beta-OH-DHEA is a metabolite [5]. The major metabolite produced in humans is a mono-hydroxylated DHEA species [4]. Synthesis of a 7alpha-hydroxy-DHEA and 5-androstene-3beta, 17beta-diol occurs in the frontal cortex, hippocampus, aμgdala, cerebellum and striatum [6].