Neutrophils Antikörper

Produktnummer ABIN1169411

Der Ratte Monoklonal Anti-Neutrophils-Antikörper wurde für FACS, IHC, Func und ICC validiert. Er ist geeignet, Neutrophils in Proben von Maus zu detektieren. Es sind 8+ Publikationen verfügbar.

Kurzübersicht für Neutrophils Antikörper (ABIN1169411)

Target: Neutrophils

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Neutrophils Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Functional Studies (Func), Immunocytochemistry (ICC)

Klon: Nimp-R14

Produktdetails anti-Neutrophils Antikörper

Spezifität: Recognizes mouse neutrophils (surface protein mouse Ly-6G) and may recognize Ly-6Chigh cells (inflammatory monocytes).

Kreuzreaktivität: Maus

Aufreinigung: Purified from concentrated hybridoma tissue culture supernatant.

Reinheit: >95 % (SDS-PAGE)

Endotoxin-Niveau: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogen: Purified mouse BALB/c neutrophils.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Negative control: AG-35B-0011 http://www.adipogen.com/ag-35b-0011/rat-igg2b-isotype-control.html

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Referenzen für anti-Neutrophils Antikörper (ABIN1169411)

Yoshida, Taguchi, Kawana, Ogishima, Adachi, Kawata, Nakamura, Sato, Fujimoto, Inoue, Tomio, Mori, Nagamatsu, Arimoto, Koga, Hiraike, Oda, Kiyono, Osuga, Fujii: "Intraperitoneal neutrophils activated by KRAS-induced ovarian cancer exert antitumor effects by modulating adaptive immunity." in: International journal of oncology, Vol. 53, Issue 4, pp. 1580-1590, (2018) (PubMed).

Henderson, Hobbs, Mathies, Hogg: "Rapid recruitment of inflammatory monocytes is independent of neutrophil migration." in: Blood, Vol. 102, Issue 1, pp. 328-35, (2003) (PubMed).

de Vries, Köhl, Leclercq, Wolfs, van Bijnen, Heeringa, Buurman: "Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 7, pp. 3883-9, (2003) (PubMed).

Tacchini-Cottier, Zweifel, Belkaid, Mukankundiye, Vasei, Launois, Milon, Louis: "An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 5, pp. 2628-36, (2000) (PubMed).

Al-Qaoud, Pearlman, Hartung, Klukowski, Fleischer, Hoerauf: "A new mechanism for IL-5-dependent helminth control: neutrophil accumulation and neutrophil-mediated worm encapsulation in murine filariasis are abolished in the absence of IL-5." in: International immunology, Vol. 12, Issue 6, pp. 899-908, (2000) (PubMed).

Lubberts, Joosten, van Den Bersselaar, Helsen, Bakker, van Meurs, Graham, Richards, van Den Berg: "Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 8, pp. 4546-56, (1999) (PubMed).

Van Lent, Blom, Holthuysen, Jacobs, Van De Putte, Van Den Berg: "Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritis in mice." in: Journal of leukocyte biology, Vol. 61, Issue 3, pp. 267-78, (1997) (PubMed).

Lopez, Strath, Sanderson: "Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies." in: British journal of haematology, Vol. 57, Issue 3, pp. 489-94, (1984) (PubMed).

Details zu Neutrophils

Target: Neutrophils

Hintergrund: Neutrophils are key components of the inflammatory response and as such contribute to the killing of microorganisms. In addition, recent evidence suggests their involvement in the development of the immune response. The Nimp-R14 mAb displays features of an optimal reagent allowing the selective depletion of mouse neutrophils in vivo.

UniProt: P35461