Tubulin-GTP Antikörper

Details zu Produkt Nr. ABIN1169388, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Fruchtfliege (Drosophila melanogaster), Human, Maus, Ratte (Rattus)
1
Wirt
Human
1
Antikörpertyp
Recombinant Antibody
Klonalität (Klon)
Monoklonal ()
Applikation
Immunocytochemistry (ICC)
1
Optionen
Hersteller
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Immunogen Full length GTP-lambda-S loaded tubulin from pig brain.
Klon MB11
Isotyp IgG2
Spezifität Recognizes human, mouse, rat and drosophila tubulin-GTP.
Kreuzreaktivität Fruchtfliege (Drosophila melanogaster), Human, Maus, Ratte (Rattus)
Reinigung Purified from HEK 293 cell culture supernatant.
Reinheit >95 % (SDS-PAGE)
Hintergrund Anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer In PBS containing 10 % glycerol and 0.02 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
Haltbarkeit 12 months
Produkt verwendet in: Nakata, Niwa, Okada, Perez, Hirokawa: "Preferential binding of a kinesin-1 motor to GTP-tubulin-rich microtubules underlies polarized vesicle transport." in: The Journal of cell biology, Vol. 194, Issue 2, pp. 245-55, 2011 (PubMed).

Dimitrov, Quesnoit, Moutel, Cantaloube, Poüs, Perez: "Detection of GTP-tubulin conformation in vivo reveals a role for GTP remnants in microtubule rescues." in: Science (New York, N.Y.), Vol. 322, Issue 5906, pp. 1353-6, 2008 (PubMed).

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