Rab1-GTP Antikörper

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Antigen
Reaktivität
Hund, Human, Maus, Ratte (Rattus)
1
Wirt
Human
1
Antikörpertyp
Recombinant Antibody
Klonalität (Klon)
Monoklonal ()
Applikation
Immunocytochemistry (ICC), Immunoprecipitation (IP)
1
1
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Immunogen Full length canine Rab1.
Klon ROF7
Isotyp IgG2
Spezifität Recognizes human, mouse, rat and dog Rab1a-GTP and Rab1b-GTP.
Kreuzreaktivität Hund, Human, Maus, Ratte (Rattus)
Reinigung Purified from HEK 293 cell culture supernatant.
Reinheit >95 % (SDS-PAGE)
Hintergrund Rab1 (Ypt1 in yeast) is a small GTPase that plays a well-established role in mediating ER-to-Golgi protein transport in both yeast and mammalian cells. Rab1 recruits effector proteins to budding COPII vesicles at the ER, forming cis-SNARE complexes that promote targeting to and fusion of these vesicles with the cis-Golgi. Rab1 is also involved in COPI vesicle formation and other distinct transport pathways, including ER-to-Golgi intermediate compartment (ERGIC)-to-cell periphery trafficking. Recently Rab1 has been shown to function in antimicrobial autophagy (autophagosome formation), as well as other forms of autophagy in mammalian cells in a way independent of ER-to-Golgi trafficking. anti-Rab1-GTP, monoclonal antibody (recombinant) (ROF7) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
UniProt P62822
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

anti-Rab1-GTP, monoclonal antibody (recombinant) (ROF7) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer In PBS containing 10 % glycerol and 0.02 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
Haltbarkeit 12 months
Produkt verwendet in: Vielemeyer, Nizak, Jimenez, Echard, Goud, Camonis, Rain, Perez: "Characterization of single chain antibody targets through yeast two hybrid." in: BMC biotechnology, Vol. 10, pp. 59, 2010 (PubMed).

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