Rekombinanter Golgin B1 (GOLGB1) Antikörper

Produktnummer ABIN1169059

Dieses Human Monoklonal-Antikörper erkennt spezifisch in ICC. Er zeigt eine Reaktivität gegenüber Human und Maus und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für Rekombinanter Golgin B1 (GOLGB1) Antikörper (ABIN1169059)

Target: Golgin B1 (GOLGB1)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus

Wirt: Human

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunocytochemistry (ICC)

Klon: TA10

Produktdetails

Spezifität: Recognizes human and mouse giantin.

Kreuzreaktivität: Human, Maus

Aufreinigung: Purified from HEK 293 cell culture supernatant.

Reinheit: >95 % (SDS-PAGE)

Immunogen: Human recombinant giantin.

Isotyp: IgG2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

anti-Giantin, monoclonal antibody (recombinant) (TA10) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-Giantin, monoclonal antibody (recombinant) (TA10) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Referenzen

Vielemeyer, Nizak, Jimenez, Echard, Goud, Camonis, Rain, Perez: "Characterization of single chain antibody targets through yeast two hybrid." in: BMC biotechnology, Vol. 10, pp. 59, (2010) (PubMed).

Nizak, Martin-Lluesma, Moutel, Roux, Kreis, Goud, Perez: "Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo." in: Traffic (Copenhagen, Denmark), Vol. 4, Issue 11, pp. 739-53, (2003) (PubMed).

Antigendetails

Target: Golgin B1 (GOLGB1)

Andere Bezeichnung: Giantin

Hintergrund: Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. It is also an antigen in chronic rheumatoid arthritis and in the autoimmune disease Sjoegren syndrome.

UniProt: Q14789

Pathways: SARS-CoV-2 Protein Interaktom