NAMPT Antikörper

Produktnummer ABIN1169028

Zitiert in 7+ Publikationen. Der Maus Monoklonal anti-NAMPT Antikörper (Klon OMNI379) (ABIN1169028) detektiert spezifisch NAMPT in WB, IHC, ELISA, IP, ICC und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Maus.

Kurzübersicht für NAMPT Antikörper (ABIN1169028)

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NAMPT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: OMNI379

Produktdetails anti-NAMPT Antikörper

Spezifität: Recognizes human, mouse and rat Nampt.

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Sterilität: 0.2 μm filtered

Immunogen: Recombinant human Nampt (visfatin/PBEF).

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Referenzen für anti-NAMPT Antikörper (ABIN1169028)

Audrito, Serra, Brusa, Mazzola, Arruga, Vaisitti, Coscia, Maffei, Rossi, Wang, Inghirami, Rizzi, Gaidano, Garcia, Wolberger, Raffaelli, Deaglio: "Extracellular nicotinamide phosphoribosyltransferase (NAMPT) promotes M2 macrophage polarization in chronic lymphocytic leukemia." in: Blood, Vol. 125, Issue 1, pp. 111-23, (2015) (PubMed).

Yoon, Yoshida, Johnson, Takikawa, Usui, Tobe, Nakagawa, Yoshino, Imai: "SIRT1-Mediated eNAMPT Secretion from Adipose Tissue Regulates Hypothalamic NAD+ and Function in Mice." in: Cell metabolism, Vol. 21, Issue 5, pp. 706-17, (2015) (PubMed).

Kover, Tong, Watkins, Clements, Stehno-Bittel, Novikova, Bittel, Kibiryeva, Stuhlsatz, Yan, Ye, Moore: "Expression and regulation of nampt in human islets." in: PLoS ONE, Vol. 8, Issue 3, pp. e58767, (2013) (PubMed).

Garten, Petzold, Barnikol-Oettler, Körner, Thasler, Kratzsch, Kiess, Gebhardt: "Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is constitutively released from human hepatocytes." in: Biochemical and biophysical research communications, Vol. 391, Issue 1, pp. 376-81, (2010) (PubMed).

Körner, Garten, Blüher, Tauscher, Kratzsch, Kiess: "Molecular characteristics of serum visfatin and differential detection by immunoassays." in: The Journal of clinical endocrinology and metabolism, Vol. 92, Issue 12, pp. 4783-91, (2007) (PubMed).

Revollo, Körner, Mills, Satoh, Wang, Garten, Dasgupta, Sasaki, Wolberger, Townsend, Milbrandt, Kiess, Imai: "Nampt/PBEF/Visfatin regulates insulin secretion in beta cells as a systemic NAD biosynthetic enzyme." in: Cell metabolism, Vol. 6, Issue 5, pp. 363-75, (2007) (PubMed).

Bae, Kim, Kim, Kim, Koo, Jang, Yun, Yoo, Bae: "Hypoxic induction of human visfatin gene is directly mediated by hypoxia-inducible factor-1." in: FEBS letters, Vol. 580, Issue 17, pp. 4105-13, (2006) (PubMed).

Details zu NAMPT

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Andere Bezeichnung: Nampt (Visfatin/PBEF)

Hintergrund: Nicotinamide phosphoribosyltransferase (Nampt, pre-B cell colony-enhancing factor, PBEF, Visfatin) is an 52 kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

UniProt: P43490