NAMPT Antikörper

Produktnummer ABIN1169028

Dieses Anti-NAMPT-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von NAMPT in WB, ELISA, IHC, IP, ICC und FACS. Geeignet für Human, Ratte und Maus. Dieses Primary Antibody wurde in 7+ Publikationen zitiert.

Kurzübersicht für NAMPT Antikörper (ABIN1169028)

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NAMPT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: OMNI379

Produktdetails anti-NAMPT Antikörper

Spezifität: Recognizes human, mouse and rat Nampt.

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Sterilität: 0.2 μm filtered

Immunogen: Recombinant human Nampt (visfatin/PBEF).

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Referenzen für anti-NAMPT Antikörper (ABIN1169028)

Audrito, Serra, Brusa, Mazzola, Arruga, Vaisitti, Coscia, Maffei, Rossi, Wang, Inghirami, Rizzi, Gaidano, Garcia, Wolberger, Raffaelli, Deaglio: "Extracellular nicotinamide phosphoribosyltransferase (NAMPT) promotes M2 macrophage polarization in chronic lymphocytic leukemia." in: Blood, Vol. 125, Issue 1, pp. 111-23, (2015) (PubMed).

Yoon, Yoshida, Johnson, Takikawa, Usui, Tobe, Nakagawa, Yoshino, Imai: "SIRT1-Mediated eNAMPT Secretion from Adipose Tissue Regulates Hypothalamic NAD+ and Function in Mice." in: Cell metabolism, Vol. 21, Issue 5, pp. 706-17, (2015) (PubMed).

Kover, Tong, Watkins, Clements, Stehno-Bittel, Novikova, Bittel, Kibiryeva, Stuhlsatz, Yan, Ye, Moore: "Expression and regulation of nampt in human islets." in: PLoS ONE, Vol. 8, Issue 3, pp. e58767, (2013) (PubMed).

Garten, Petzold, Barnikol-Oettler, Körner, Thasler, Kratzsch, Kiess, Gebhardt: "Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is constitutively released from human hepatocytes." in: Biochemical and biophysical research communications, Vol. 391, Issue 1, pp. 376-81, (2010) (PubMed).

Körner, Garten, Blüher, Tauscher, Kratzsch, Kiess: "Molecular characteristics of serum visfatin and differential detection by immunoassays." in: The Journal of clinical endocrinology and metabolism, Vol. 92, Issue 12, pp. 4783-91, (2007) (PubMed).

Revollo, Körner, Mills, Satoh, Wang, Garten, Dasgupta, Sasaki, Wolberger, Townsend, Milbrandt, Kiess, Imai: "Nampt/PBEF/Visfatin regulates insulin secretion in beta cells as a systemic NAD biosynthetic enzyme." in: Cell metabolism, Vol. 6, Issue 5, pp. 363-75, (2007) (PubMed).

Bae, Kim, Kim, Kim, Koo, Jang, Yun, Yoo, Bae: "Hypoxic induction of human visfatin gene is directly mediated by hypoxia-inducible factor-1." in: FEBS letters, Vol. 580, Issue 17, pp. 4105-13, (2006) (PubMed).

Details zu NAMPT

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Andere Bezeichnung: Nampt (Visfatin/PBEF)

Hintergrund: Nicotinamide phosphoribosyltransferase (Nampt, pre-B cell colony-enhancing factor, PBEF, Visfatin) is an 52 kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

UniProt: P43490