Rekombinanter Myosin 9 Antikörper

Produktnummer ABIN1169007

Der Human Monoklonal anti-Myosin 9 Antikörper (Klon SF9) (ABIN1169007) detektiert spezifisch Myosin 9 in WB, ELISA und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Drosophila melanogaster.

Kurzübersicht für Rekombinanter Myosin 9 Antikörper (ABIN1169007)

Target: Myosin 9 (MYH9)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte, Drosophila melanogaster

Wirt: Human

Klonalität: Monoklonal

Konjugat: Dieser Myosin 9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Klon: SF9

Produktdetails anti-Myosin 9 Antikörper

Verwendungszweck: anti-Myosin IIA (non-muscle) (heavy chain), mAb (rec.) (SF9)

Spezifität: Recognizes human, mouse, rat and drosophila myosin IIA (heavy chain).

Produktmerkmale: Recombinant Antibody. Recognizes human, mouse, rat and drosophila myosin IIA (heavy chain). Species cross-reactivity: Drosophila, Human, Mouse, Rat. Clone: SF9. Isotype: Human IgG2lambda. Applications: ELISA, ICC, WB. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Aufreinigung: Purified from HEK 293 cell culture supernatant.

Reinheit: >95 % (SDS-PAGE)

Immunogen: Full length myosin IIA from rat liver.

Isotyp: IgG2 lambda

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

+4°C

-20°C

Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Details zu Myosin 9

Target: Myosin 9 (MYH9)

Andere Bezeichnung: Myosin IIA

Hintergrund:

Alternate Names/Synonyms: Cellular Myosin Heavy Chain, Type A, Myosin Heavy Chain 9, Myosin Heavy Chain, Non-muscle IIa, Non-muscle Myosin Heavy Chain A

Product Description: anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

UniProt: P35579

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Integrin Complex