Luciferase Antikörper

Produktnummer ABIN116764

Dieser Ziege Polyklonal Antikörper detektiert spezifisch Luciferase in WB, IP, IHC (fro) und EIA. Es zeigt Reaktivität gegenüber Proben von Photinus pyralis.

Kurzübersicht für Luciferase Antikörper (ABIN116764)

Target: Luciferase

Reaktivität: Photinus pyralis

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Luciferase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Produktdetails anti-Luciferase Antikörper

Aufreinigung: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Luciferase [Firefly].

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation,Immunhistochemistry and most Immunological methods requiring high titer andspecificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Luciferase[Firefly] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature. A working dilution of 1: 20000 to1: 100000 of the reconstitution concentration is suggested for this product. For Western Blot, a recommended working dilution is 1: 2000 - 1: 10000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 95.0 mg/mL (by Refractometry)

Buffer: PBS, pH 7.2 containing 0.01 % sodium azides as a preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Details zu Luciferase

Target: Luciferase

Hintergrund: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.Synonyms: Luciferin 4-monooxygenase

Gen-ID: 7054

UniProt: P08659