Luciferase Antikörper

Produktnummer ABIN116764

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Luciferase in WB, IP, IHC (fro) und EIA. Er zeigt eine Reaktivität gegenüber Photinus pyralis.

Kurzübersicht für Luciferase Antikörper (ABIN116764)

Target: Luciferase

Reaktivität: Photinus pyralis

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Luciferase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Produktdetails anti-Luciferase Antikörper

Aufreinigung: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Luciferase [Firefly].

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation,Immunhistochemistry and most Immunological methods requiring high titer andspecificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Luciferase[Firefly] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature. A working dilution of 1: 20000 to1: 100000 of the reconstitution concentration is suggested for this product. For Western Blot, a recommended working dilution is 1: 2000 - 1: 10000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 95.0 mg/mL (by Refractometry)

Buffer: PBS, pH 7.2 containing 0.01 % sodium azides as a preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Details zu Luciferase

Target: Luciferase

Hintergrund: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.Synonyms: Luciferin 4-monooxygenase

Gen-ID: 7054

UniProt: P08659