Der Kaninchen Polyklonal anti-Glucose Oxidase Antikörper wird verwendet zum Nachweis von Glucose Oxidase in Proben von Aspergillus. Er wurde validiert für WB, IP und EIA.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Glucose Oxidase[Aspergillus niger] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Rabbit IgG [H&L] (Rabbit) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 4,000 to 1: 16,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore with 2.0 mL of deionized water (or equivalent).
Konzentration
90.0 mg/mL (by Refractometry)
Buffer
0.01 M Sodium phosphate, 0.15 M Sodium chloride, pH 7.2 containing no preservatives/stabilizers
Konservierungsmittel
Without preservative
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store vial at -20 °C prior to opening. Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
Glucose Oxidase (GOD)
Andere Bezeichnung
Glucose Oxidase
Hintergrund
Glucose Oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose or oxygen from foods and beverages. Glucose Oxidase producing moulds such as Aspergillus and Penicillium species are used for the biological production of gluconic acid. CATALYTIC ACTIVITY: Beta D glucose + O2 = D glucono 1,5 lactone + H2O2.Synonyms: Beta-D-glucose:oxygen 1-oxido-reductase, Glucose oxyhydrase, gox