Glucose-6-Phosphate Dehydrogenase Antikörper

Produktnummer ABIN116713

Dieser Ziege Polyklonal Antikörper detektiert spezifisch Glucose-6-Phosphate Dehydrogenase in WB und EIA. Es zeigt Reaktivität gegenüber Proben von Bacillus.

Kurzübersicht für Glucose-6-Phosphate Dehydrogenase Antikörper (ABIN116713)

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reaktivität: Bacillus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Glucose-6-Phosphate Dehydrogenase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Glucose-6-Phosphate Dehydrogenase Antikörper

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glucose Dehydrogenase [Bacillus]. Cross reactivity against Glucose Dehydrogenase from other sources is unknown.

Aufreinigung: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Glucose Dehydrogenase [Bacillus].

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of GlucoseDehydrogenase [Bacillus] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Goat IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 7,500 to 1: 32,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C prior to opening. For extended storage, aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.

Details zu Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Andere Bezeichnung: Glucose Dehydrogenase

Hintergrund: Glucose Dehydrogenase catalyses the oxidation of D glucose without prior phosphorylation to D beta gluconolactone using NAD or NADP as a coenzyme. The enzyme is a tetrameric protein, each of the 4 identical subunits containing 262 amino acid residues. This family is a subset of a more general family of short chain dehydrogenases and reductases.Synonyms: BSU03930, Glucose 1-dehydrogenase, gdh

Gen-ID: 938261

NCBI Accession: NP_388275

UniProt: P12310

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones