Cholesterol Oxidase Antikörper

Produktnummer ABIN116685

Der Ziege Polyklonal anti-Cholesterol Oxidase Antikörper (ABIN116685) detektiert spezifisch Cholesterol Oxidase in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Bakterien.

Kurzübersicht für Cholesterol Oxidase Antikörper (ABIN116685)

Target: Cholesterol Oxidase (choS)

Reaktivität: Bakterien

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Cholesterol Oxidase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Cholesterol Oxidase Antikörper

Aufreinigung: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Cholesterol Oxidase [Microorganism].

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayed against 1.0 μg of Cholesterol Oxidase [Microorganism] in astandard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L](Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for30 minutes at room temperature. A working dilution of 1: 8,000 to 1: 40,000 of thereconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Details zu Cholesterol Oxidase

Target: Cholesterol Oxidase (choS)

Andere Bezeichnung: Cholesterol Oxidase

Hintergrund: Cholesterol Oxidases exist as both type I and type II oxidases and are implicated in bacterial pathogenesis. In addition, they are important as clinical reagents, potential larvicides, and tools in cell biology.Synonyms: CHOD, Cholesterol oxidase, EC 1.1.3.6