Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
Spezifität
This antibody detects porcine Carbxypeptidase B. Cross reactivity against Carboxypeptidase B from other tissues and species may occur but have not been specifically determined.Immunoelectrophoresis give a single precipitin arc against purified and partially purified Carboxypeptidase B [porcine pancreas].
Aufreinigung
Prepared from monospecific antiserum by a delipidation and defibrination.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostImmunological methods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/2,000-1/10,000. Western Blot: 1/500-1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore with 2.0 mL of deionized water (or equivalent).
Konzentration
90.0 mg/mL (by Refractometry)
Buffer
0.02 M Potassium phosphate, 0.12 M Sodium chloride, pH 7.2, 0.01 % (w/v) Sodium azide, None
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
Carboxypeptidase catalyzes the hydrolysis of the basic amino acids lysine, arginine and ornithine from the C terminal position in polypeptides. It occurs as a zymogen in the pancreas in most vertebrates.Synonyms: CPB, CPB1, PASP, PCPB, Pancreas-specific protein