Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
Spezifität
This antibody detects Carbonic anhydrase 2. Immunoelectrophoresis give a single precipitin arc against purified and partially purified Carbonic anhydrase 2 [bovine erythrocytes].
Aufreinigung
This product was prepared from monospecific antiserum by delipidation and defibrination.
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayed against 1.0 μg of Carbonic Anhydrase II [BovineErythrocytes] in a standard sandwich ELISA using Peroxidase conjugated affinity purifiedanti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 3,000 to1: 14,000 of the reconstitution concentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore with 2.0 mL of deionized water (or equivalent).
Konzentration
85.0 mg/mL (by refractometry)
Buffer
0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2, 0.01 % (w/v) Sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Dilute only prior to immediate use
Lagerung
4 °C
Informationen zur Lagerung
Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid.
Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29 kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20μM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.Synonyms: CA-II, CA2, CAC, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II