CA2 Antikörper

Produktnummer ABIN116657

Der Kaninchen Polyklonal anti-CA2 Antikörper wird verwendet zum Nachweis von CA2 in Proben von Human. Er wurde validiert für WB und EIA.

Kurzübersicht für CA2 Antikörper (ABIN116657)

Target: CA2 (Carbonic Anhydrase II (CA2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CA2 Antikörper

Spezifität: This antibody detects human Carbonic anhydrase 2. Cross reactivity against Carbonic anhydrase 2 from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against purified and partially purified Carbonic anhydrase 2 [human erythrocytes].

Aufreinigung: Prepared from monospecific antiserum by a delipidation and defibrination

Immunogen: Carbonic Anhydrase II from Human Erythrocytes. Genename: CA2

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA (1/115,000) and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of CarbonicAnhydrase II [Human Erythrocytes] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) R1364HRP and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 1,000 to 1: 5,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 80.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium phosphate, 0.12 M Sodium chloride, pH 7.2, 0.01 % (w/v) Sodium azide, None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Prior to and following reconstitution store undiluted at 2-8 °C. DO NOT FREEZE! This antibody should be protected from light as the PE-labelled product is photosensitive.

Details zu CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Andere Bezeichnung: Carbonic Anhydrase 2

Hintergrund: Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29 kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20μM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.Synonyms: CA-II, CA2, CAC, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II

Gen-ID: 760

NCBI Accession: NP_000058

UniProt: P00918