L-Asparaginase 1 Antikörper

Produktnummer ABIN116628

Produktdetails

Target: L-Asparaginase 1

Reaktivität: E. coli

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Asparaginase [Escherichia coli]. Cross reactivity against Asparaginase from other tissues and species may occur but have not been specifically determined.

Aufreinigung: Prepared from monospecific antiserum by Delipidation and Defibrination.

Immunogen: Asparaginase [Escherichia coli].

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended DilutionsELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with 2.0 mL of deionized water or equivalent.

Konzentration: 65.0 mg/mL (by Refractometry)

Buffer: 0.02M Sodium Phosphate, 0.15M Sodium Chloride, pH 7.2 without preservatives or stabilizers.

Konservierungsmittel: Without preservative

Handhabung: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20°C. For extended storage aliquot contents and freeze at -20°C or below.

Haltbarkeit: 12 months

Antigendetails

Target: L-Asparaginase 1

Hintergrund: Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.
Alternate names: L-ASNase I, L-asparaginase I, L-asparagine amidohydrolase I, ansA

Gen-ID: 946278

NCBI Accession: AP_002386

UniProt: P0A962