ALDOA Antikörper

Produktnummer ABIN116608

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch ALDOA in WB und EIA. Er zeigt eine Reaktivität gegenüber Human und Kaninchen.

Kurzübersicht für ALDOA Antikörper (ABIN116608)

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Reaktivität: Human, Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ALDOA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-ALDOA Antikörper

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Aldolase [Rabbit Muscle]. This antibody will detect human Aldolase. Cross reactivity against Aldolase from other tissues and species may also occur. It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines.

Aufreinigung: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Aldolase [Rabbit Muscle].

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostImmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 m g of Aldolase[Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG [H&L] (Rabbit) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 3,000 to1: 12,000 of the reconstitution concentration is suggested for this product. Useapproximately 5 μL of antibody to immunoprecipitate 50 μL of protein lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Details zu ALDOA

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Hintergrund: Synonyms: ALDA, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, NY-LU-1

Gen-ID: 100009055

NCBI Accession: NP_001075707

UniProt: P00883

Pathways: Ribonucleoside Biosynthetic Process