Intestinal Alkaline Phosphatase Antikörper

Produktnummer ABIN116600

Der Kaninchen Polyklonal anti-Intestinal Alkaline Phosphatase Antikörper wird verwendet zum Nachweis von Intestinal Alkaline Phosphatase in Proben von Human. Er wurde validiert für WB und EIA.

Kurzübersicht für Intestinal Alkaline Phosphatase Antikörper (ABIN116600)

Target: Intestinal Alkaline Phosphatase (ALPI)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Intestinal Alkaline Phosphatase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Intestinal Alkaline Phosphatase Antikörper

Spezifität: This antibody detects human Alkaline phosphatase. Cross reactivity against Alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline phosphatase [human intestine].

Aufreinigung: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Alkaline Phosphatase [Human Intestine].

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA and most immunological methodsrequiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of AlkalinePhosphatase [Human Intestine] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 5,000 to 1: 20,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 85.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassiumphosphate, 0.15 M Sodium chloride, pH 7.2 with 0.01 % (w/v) Sodium azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Details zu Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Andere Bezeichnung: Alkaline Phosphatase / ALPI / IAP

Hintergrund: Synonyms: Intestinal alkaline phosphatase

Gen-ID: 248

NCBI Accession: NP_001622

UniProt: P09923