Alkaline Phosphatase Antikörper

Produktnummer ABIN116596

Produktdetails anti-Alkaline Phosphatase Antikörper

Target: Alkaline Phosphatase (ALP)

Reaktivität: E. coli

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Alkaline Phosphatase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Spezifität: This product was prepared from monospecific antiserum by a Delipidation and Defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli]. No cross reactivity occurs against the mammalian form of Alkaline Phosphatase.

Aufreinigung: Delipidation and Defibrination

Immunogen: Alkaline Phosphatase [E.coli].

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostImmunological methods requiring high titer and specificity. Note: This product has been assayed against 1.0 μg of Alkaline Phosphatase [E. coli] in astandard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG[H&L] (Goat) (Cat#R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to1/100,000 of the reconstitution concentration is suggested for this product. Recommended Dilutions: ELISA: 1/8,000-1/40,000Western Blot: 1/500-1/2,000
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 75.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below.

Details zu Alkaline Phosphatase

Target: Alkaline Phosphatase (ALP)

Andere Bezeichnung: Alkaline Phosphatase (ALP Produkte)

Synonyme: ALP antikoerper, ALP1 antikoerper, CRASH antikoerper, ECK0378 antikoerper, JW0374 antikoerper, psiA antikoerper, 2410004D18Rik antikoerper, AU040643 antikoerper, AW060375 antikoerper, ILC antikoerper, CTACK antikoerper, ESkine antikoerper, Akp2 antikoerper, alp antikoerper, zgc:56672 antikoerper, DDBDRAFT_0205491 antikoerper, DDBDRAFT_0231570 antikoerper, DDB_0205491 antikoerper, DDB_0231570 antikoerper, Actn2lp antikoerper, Alp antikoerper, asparaginase like 1 antikoerper, bacterial alkaline phosphatase antikoerper, C-C motif chemokine ligand 27 antikoerper, alkaline phosphatase, liver/bone/kidney antikoerper, alkaline phosphatase antikoerper, PDZ and LIM domain 3 antikoerper, ASRGL1 antikoerper, phoA antikoerper, Asrgl1 antikoerper, CCL27 antikoerper, alpl antikoerper, alp antikoerper, Pdlim3 antikoerper

Hintergrund: Synonyms: JW0374, b0383, phoA

Gen-ID: 945041

NCBI Accession: AP_001034

UniProt: P00634