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NFYA Antikörper (N-Term)

NFYA Reaktivität: Human, Maus WB, IP, EMSA, EIA Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN116536
  • Target Alle NFYA Antikörper anzeigen
    NFYA (Nuclear Transcription Factor Y, alpha (NFYA))
    Bindungsspezifität
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    N-Term
    Reaktivität
    • 26
    • 21
    • 18
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    Human, Maus
    Wirt
    • 26
    Kaninchen
    Klonalität
    • 24
    • 2
    Polyklonal
    Konjugat
    • 19
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser NFYA Antikörper ist unkonjugiert
    Applikation
    • 17
    • 11
    • 7
    • 7
    • 7
    • 5
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA)
    Spezifität
    This product was prepared from monospecific antiserum by a multi-step procedure that includes delipidation, salt fractionation and ion exchange chromatography. A single precipitin arc was observed against anti-Rabbit Serum when assayed by Immunoelectrophoresis. No reactivity was observed by ELISA against the B subunit of NFY.
    Aufreinigung
    Multistep process.
    Immunogen
    NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
    Top Product
    Discover our top product NFYA Primärantikörper
  • Applikationshinweise
    Suitable for Immunoprecipitation, Immunoblotting, ELISA and supershift assays. Recommended Dilution(s): This product was assayed by Immunoblot and found to be reactive against thedifferentially spliced 35 and 42 kDa forms of the A subunit of human and mouse NF-Y at adilution of 1: 1000 followed by reaction with Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP). No reaction was observed by immunoblotagainst the 25 kDa B subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive againsthuman and mouse NF-Y using 2.0 μL per assay. This product was assayed against NF-Y A subunit peptide in an antibody capture ELISAusing Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP)and ABTS substrate. A dilution of 1: 10,000 is suggested from this experiment.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.1 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % Sodium Azide as preservative.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store lyophilized (preferably in a desiccator) at 2-8 °C and reconstituted (in aliquots) at -20 °C.
  • Target
    NFYA (Nuclear Transcription Factor Y, alpha (NFYA))
    Andere Bezeichnung
    NFYA (NFYA Produkte)
    Synonyme
    wu:fb16e03 antikoerper, ik:tdsubc_2b6 antikoerper, xx:tdsubc_2b6 antikoerper, NFYA antikoerper, CBF-A antikoerper, CBF-B antikoerper, HAP2 antikoerper, NF-YA antikoerper, AA407810 antikoerper, Cbf-b antikoerper, SEZ-10 antikoerper, SEZ10 antikoerper, nuclear transcription factor Y, alpha antikoerper, nuclear transcription factor Y subunit alpha antikoerper, nuclear transcription factor-Y alpha antikoerper, nfya antikoerper, NFYA antikoerper, Nfya antikoerper
    Hintergrund
    NFY is a transcription factor composed of 3 separate subunits (A,B and C) each of which is required for DNA binding. NFY binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. NFY recognizes a CCAAT motif upstream of gene promoters and is probably involved in the regulation of a variety of genes. Each subunit has remained highly conserved throughout evolution. In fact, homologous yeast subunits can substitute for mammalian NFY in DNA binding assays. The conserved core sequences of NFYB and NFYC contain a 70 aa region similar to the histone fold motif of nucleosomes H2A and H2B. The unique structure and evolutionary conservation of this transcription factor suggests that it plays a fundamental role in the readout of eukaryotic genetic information.Synonyms: Nuclear transcription factor Y subunit alpha
    Gen-ID
    4800
    NCBI Accession
    NP_002496
    UniProt
    P23511
    Pathways
    Regulation of Lipid Metabolism by PPARalpha
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