NFYA Antikörper (N-Term)

Produktnummer ABIN116536

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NFYA in WB, IP, EMSA und EIA. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für NFYA Antikörper (N-Term) (ABIN116536)

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFYA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA)

Produktdetails anti-NFYA Antikörper

Bindungsspezifität: N-Term

Spezifität: This product was prepared from monospecific antiserum by a multi-step procedure that includes delipidation, salt fractionation and ion exchange chromatography. A single precipitin arc was observed against anti-Rabbit Serum when assayed by Immunoelectrophoresis. No reactivity was observed by ELISA against the B subunit of NFY.

Aufreinigung: Multistep process.

Immunogen: NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoprecipitation, Immunoblotting, ELISA and supershift assays. Recommended Dilution(s): This product was assayed by Immunoblot and found to be reactive against thedifferentially spliced 35 and 42 kDa forms of the A subunit of human and mouse NF-Y at adilution of 1: 1000 followed by reaction with Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP). No reaction was observed by immunoblotagainst the 25 kDa B subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive againsthuman and mouse NF-Y using 2.0 μL per assay. This product was assayed against NF-Y A subunit peptide in an antibody capture ELISAusing Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP)and ABTS substrate. A dilution of 1: 10,000 is suggested from this experiment.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at 2-8 °C and reconstituted (in aliquots) at -20 °C.

Details zu NFYA

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Andere Bezeichnung: NFYA

Hintergrund: NFY is a transcription factor composed of 3 separate subunits (A,B and C) each of which is required for DNA binding. NFY binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. NFY recognizes a CCAAT motif upstream of gene promoters and is probably involved in the regulation of a variety of genes. Each subunit has remained highly conserved throughout evolution. In fact, homologous yeast subunits can substitute for mammalian NFY in DNA binding assays. The conserved core sequences of NFYB and NFYC contain a 70 aa region similar to the histone fold motif of nucleosomes H2A and H2B. The unique structure and evolutionary conservation of this transcription factor suggests that it plays a fundamental role in the readout of eukaryotic genetic information.Synonyms: Nuclear transcription factor Y subunit alpha

Gen-ID: 4800

NCBI Accession: NP_002496

UniProt: P23511

Pathways: Regulation of Lipid Metabolism by PPARalpha