Cyclin T1 Antikörper (Internal Region)

Produktnummer ABIN116513

Der Kaninchen Polyklonal Anti-Cyclin T1-Antikörper wurde für WB, IP, IHC (p), EIA und IHC (fro) validiert. Er ist geeignet, Cyclin T1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Cyclin T1 Antikörper (Internal Region) (ABIN116513)

Target: Cyclin T1 (CCNT1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin T1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Cyclin T1 Antikörper

Bindungsspezifität: Internal Region

Spezifität: Antiserum will specifically react with a 85 kDa cyclin T1 protein. No reaction was observed against other related cyclins.

Aufreinigung: Delipidation and defibrination

Immunogen: Cyclin T1 peptide corresponding to an internal region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Anwendungsinformationen

Applikationshinweise: Suitable for ELISA, immunoprecipitation, immunohistochemistry, immunoblotting andother immunological methods requiring high titer and specificity. Recommended dilutions: ELISA 1: 1,000 - 1: 5,000Western blot 1: 500 - 1: 2,000Immunohistochemistry 1: 200 - 1: 1,000 (use paraffin embedded tissue).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 75 mg/mL (by refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, containing 0.01 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C to -80 °C. Avoid multiple freeze/thaw cycles.

Details zu Cyclin T1

Target: Cyclin T1 (CCNT1)

Andere Bezeichnung: Cyclin T1

Hintergrund: Cyclin T1, together with the kinase CDK9, is a component of the transcription elongation factor P-TEFb which binds the human immunodeficiency virus type 1 (HIV-1) transactivator Tat. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. P-TEFb facilitates transcription by phosphorylating the carboxy-terminal domain (CTD) of RNA polymerase II. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. Cyclin T1 is an exceptionally large cyclin and is therefore a candidate for interactions with regulatory proteins.Synonyms: CCNT1, CycT1, Cyclin-T, Cyclin-T1

Gen-ID: 904

NCBI Accession: NP_001231

UniProt: O60563