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DEFB103A Antikörper (Biotin)

DEFB103A Reaktivität: Human WB, EIA Wirt: Kaninchen Polyclonal Biotin
Produktnummer ABIN116503
  • Target Alle DEFB103A Antikörper anzeigen
    DEFB103A (Defensin, beta 103A (DEFB103A))
    Reaktivität
    • 8
    • 1
    Human
    Wirt
    • 4
    • 3
    • 1
    Kaninchen
    Klonalität
    • 6
    • 2
    Polyklonal
    Konjugat
    • 8
    Dieser DEFB103A Antikörper ist konjugiert mit Biotin
    Applikation
    • 7
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Spezifität
    This antibody reacts with Human BD3 (Defensin beta 3). Other species not tested.
    Aufreinigung
    Affinity Chromatography
    Immunogen
    E.coli derived recombinant Human Beta Defensin-3.
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    Discover our top product DEFB103A Primärantikörper
  • Applikationshinweise
    ELISA: Direct: To detect Human BD-3 by direct ELISA (using 100 μL/well antibody solution) thisantibody can be used at a concentration of 1.0 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant HumanBD-3. Sandwich: To detect Human BD-3 by Sandwich ELISA (using 100 μL/well antibody solution)this antibody can be used at a concentration of 0.25-1.0 μg/mL. This biotinylated antibody,used in conjunction with polyclonal anti-human BD-3, allows the detection of at least0.2-0.4 ng/well of recombinant Human BD-3. Western blot: To detect Human BD-3 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hBD-3 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL
    Buffer
    BPS, pH 7.2 without preservatives
    Konservierungsmittel
    Without preservative
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
  • Target
    DEFB103A (Defensin, beta 103A (DEFB103A))
    Andere Bezeichnung
    Defensin beta 3 (DEFB103A Produkte)
    Synonyme
    BD-3 antikoerper, DEFB-3 antikoerper, DEFB103 antikoerper, DEFB3 antikoerper, HBD3 antikoerper, HBP-3 antikoerper, HBP3 antikoerper, hBD-3 antikoerper, DEFB103B antikoerper, defensin beta 103A antikoerper, defensin beta 103B antikoerper, defensin, beta 103A antikoerper, beta-defensin 103 antikoerper, DEFB103A antikoerper, DEFB103B antikoerper, LOC694235 antikoerper, LOC100359096 antikoerper
    Hintergrund
    Beta Defensin 3 exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. It kills multiresistant S.aureus and vancomycin-resistant E.faecium, but it appears to have no significant hemolytic activity. Beta Defensin 3 is highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression is observed in salivary gland, bone marrow, colon, stomach, polyp and larynx, with no expression in small intestine. Expression is induced by infection of bacteria and by interferon gamma. Beta Defensin 3 belongs to the beta-defensin family.Synonyms: BD3, Beta-defensin 103, DEFB103, DEFB103A, DEFB103B, DEFB3, Defensin beta 103, Defensin-like protein, HBD3, HBP3
    Gen-ID
    414325
    NCBI Accession
    NP_001075020
    UniProt
    P81534
    Pathways
    Production of Molecular Mediator of Immune Response
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