DEFB103A Antikörper (Biotin)

Produktnummer ABIN116503

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch DEFB103A in WB und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für DEFB103A Antikörper (Biotin) (ABIN116503)

Target: DEFB103A (Defensin, beta 103A (DEFB103A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DEFB103A Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-DEFB103A Antikörper (Biotin)

Spezifität: This antibody reacts with Human BD3 (Defensin beta 3). Other species not tested.

Aufreinigung: Affinity Chromatography

Immunogen: E.coli derived recombinant Human Beta Defensin-3.

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect Human BD-3 by direct ELISA (using 100 μL/well antibody solution) thisantibody can be used at a concentration of 1.0 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant HumanBD-3. Sandwich: To detect Human BD-3 by Sandwich ELISA (using 100 μL/well antibody solution)this antibody can be used at a concentration of 0.25-1.0 μg/mL. This biotinylated antibody,used in conjunction with polyclonal anti-human BD-3, allows the detection of at least0.2-0.4 ng/well of recombinant Human BD-3. Western blot: To detect Human BD-3 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hBD-3 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL

Buffer: BPS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Details zu DEFB103A

Target: DEFB103A (Defensin, beta 103A (DEFB103A))

Andere Bezeichnung: Defensin beta 3

Hintergrund: Beta Defensin 3 exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. It kills multiresistant S.aureus and vancomycin-resistant E.faecium, but it appears to have no significant hemolytic activity. Beta Defensin 3 is highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression is observed in salivary gland, bone marrow, colon, stomach, polyp and larynx, with no expression in small intestine. Expression is induced by infection of bacteria and by interferon gamma. Beta Defensin 3 belongs to the beta-defensin family.Synonyms: BD3, Beta-defensin 103, DEFB103, DEFB103A, DEFB103B, DEFB3, Defensin beta 103, Defensin-like protein, HBD3, HBP3

Gen-ID: 414325

NCBI Accession: NP_001075020

UniProt: P81534

Pathways: Production of Molecular Mediator of Immune Response