LGALS1/Galectin 1 Antikörper

Produktnummer ABIN116481

Dieses Anti-LGALS1/Galectin 1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von LGALS1/Galectin 1 in WB und EIA. Geeignet für Human.

Kurzübersicht für LGALS1/Galectin 1 Antikörper (ABIN116481)

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LGALS1/Galectin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-LGALS1/Galectin 1 Antikörper

Spezifität: Recognizes Galectin-1

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography employing immobilized Human Galectin-1 matrix.

Immunogen: E. Coli derived Recombinant Human Galectin-1.

Anwendungsinformationen

Applikationshinweise: Sandwich ELISA: To detect Human Galectin-1 (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human Galectin-1 (PP1223B1 and PP1223B2)as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinantHuman Galectin-1. Western Blot: To detect Human Galectin-1 by this analysis, the antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGalectin-1 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu LGALS1/Galectin 1

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Andere Bezeichnung: Galectin-1

Hintergrund: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin 1 may act as an autocrine negative growth factor that regulates cell proliferation. It also may regulate cell apoptosis and cell differentiation. It binds beta-galactoside, CD45, CD3 and CD4. Galectin 1 inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. It is found in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney, placenta and hematopoietic cells.Synonyms: 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, Galectin1, HBL, HPL, LGALS1, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, MAPK-activating protein PM12, S-Lac lectin 1

Molekulargewicht: approx. 150 kDa

Gen-ID: 3956

NCBI Accession: NP_002296

UniProt: P09382

Pathways: Carbohydrate Homeostasis