BMP2 Antikörper

Produktnummer ABIN116466

Produktdetails anti-BMP2 Antikörper

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BMP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Spezifität: The antibody recognizes Human BMP2. Other species not tested.

Aufreinigung: Affinity Chromatography employing immobilized Human BMP-2 matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant Human BMP-2.

Anwendungsinformationen

Applikationshinweise: NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of hBMP-2 (200 ng/mL), a concentration of 1.3-1.9 μg/mL of this antibody is required. ELISAIndirect: To detect hBMP-2 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hBMP-2. Sandwich: To detect hBMP-2 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with a biotinylated Anti-Human BMP-2 (PP1219B) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBMP-2. Western BlotTo detect hBMP-2 by Western Blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hBMP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge the vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu BMP2

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Andere Bezeichnung: BMP2 / BMP2A (BMP2 Produkte)

Synonyme: BDA2 antikoerper, BMP2A antikoerper, AI467020 antikoerper, Bmp2a antikoerper, BMP-2 antikoerper, xBMP-2 antikoerper, xbmp2 antikoerper, BMP2 antikoerper, bmp2a antikoerper, bmp2 antikoerper, wu:fc59d09 antikoerper, bone morphogenetic protein 2 antikoerper, bone morphogenetic protein 2 L homeolog antikoerper, Bone morphogenetic protein 2 antikoerper, bone morphogenetic protein 2a antikoerper, BMP2 antikoerper, Bmp2 antikoerper, bmp2.L antikoerper, bmp2 antikoerper, bmp2a antikoerper

Hintergrund: BMP2 belongs to the transforming growth factor beta (TGFB) superfamily of secreted growth factors. It is a disulfide linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis.Synonyms: BMP-2, BMP-2A, Bone morphogenetic protein 2

Gen-ID: 650

NCBI Accession: NP_001191

UniProt: P12643

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation