CXCL10 Antikörper (Biotin)

Produktnummer ABIN116269

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CXCL10 in WB und EIA. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für CXCL10 Antikörper (Biotin) (ABIN116269)

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCL10 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CXCL10 Antikörper (Biotin)

Spezifität: Reacts with Interferon-gamma Inducible Protein-10.

Aufreinigung: Affinity Chromatography

Immunogen: Highly pure (>98%) E.coli derived recombinant Mouse IP-10.

Anwendungsinformationen

Applikationshinweise: Direct ELISA: To detect Mouse IP-10 by Direct ELISA (using 100 ml/well antibody solution)this antibody can be used at a concentration of approx. 1.0 μg/mL. This Biotinylated antibodyallows the detection of at least 0.2-0.4 ng/well of recombinant Mouse IP-10. Sandwich ELISA: To detect Mouse IP-10 by Sandwich ELISA (using 100 ml/well antibodysolution), a concentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylatedantibody, in conjuction with anti Mouse IP10 antibody (cat. PP1083P) as a Captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse IP-10. Western Blot: To detect Mouse IP-10 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Mouse IP-10 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu CXCL10

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Andere Bezeichnung: CXCL10 / IP-10

Hintergrund: Interferon-gamma-inducible 10 kD protein (IP-10), is a CXC chemokine with chemoattractant properties for CD4-positive T cells and inhibits early normal and leukemic hemopoietic progenitor proliferation. IP-10 is produced by a wide variety of cell types ranging from neutrophils and monocytes to hepatocytes, endothelial cells and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV encephalitis, cutaneous T cell lymphoma, chronic hepatitis and acute anterior uveitis. Various observations strongly suggest a role for the CXC chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis.Synonyms: 10 kDa interferon-gamma-induced protein, C-X-C motif chemokine 10, Gamma-IP10, INP10, SCYB10, Small-inducible cytokine B10

Gen-ID: 15945

NCBI Accession: NP_067249

UniProt: P17515