CCL5 Antikörper (Biotin)

Produktnummer ABIN116261

Der Kaninchen Polyklonal anti-CCL5 Antikörper wird verwendet zum Nachweis von CCL5 in Proben von Maus. Er wurde validiert für WB und EIA.

Kurzübersicht für CCL5 Antikörper (Biotin) (ABIN116261)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCL5 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CCL5 Antikörper (Biotin)

Spezifität: The antibody reacts with Murine RANTES.

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Aufreinigung: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant Murine RANTES. Remarks: Molecular Weight: ~150 kDa

Anwendungsinformationen

Applikationshinweise: ELISADirect: To detect Murine RANTES by Direct ELISA (using 100 μL/well antibody solution) aconcentration of approx. 1.0 μg/mL of this antibody is required. This Biotinylated polyclonalpurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant Murine RANTES. Sandwich: To detect Murine RANTES by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylatedpolyclonal antibody, in conjunction with Anti Murine RANTES (PP1081P1 or PP1081P2) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinantmRANTES. Western BlotTo detect Murine RANTES by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mRANTES is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu CCL5

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Andere Bezeichnung: RANTES / CCL5

Hintergrund: RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.Synonyms: C-C motif chemokine 5, D17S136E, E °CP, Eosinophil-chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, cell-specific protein P228

Gen-ID: 20304

NCBI Accession: NP_038681

UniProt: P30882

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration