LTA Antikörper (Biotin)

Produktnummer ABIN116245

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch LTA in WB und EIA. Er zeigt eine Reaktivität gegenüber .

Kurzübersicht für LTA Antikörper (Biotin) (ABIN116245)

Target: LTA (Lymphotoxin-alpha (LTA))

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LTA Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-LTA Antikörper (Biotin)

Spezifität: This antibody reacts with Tumor Necrosis Factor beta.

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hTNF-beta.

Anwendungsinformationen

Applikationshinweise: ELISA: (Direct): To detect hTNF-β by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTNF-β. (Sandwich): To detect hTNF-β by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Human TNF-β (PP1072P1/PP1072P2) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTNF-β. Western Blot: To detect hTNF-β by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTNF-β is 1.5- 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu LTA

Target: LTA (Lymphotoxin-alpha (LTA))

Andere Bezeichnung: TNF-beta / Lymphotoxin-alpha

Substanzklasse: Chemical

Hintergrund: Synonyms: LT-alpha, LTA, TNFB, TNFSF1, Tumor necrosis factor ligand superfamily member 1

Gen-ID: 4049

NCBI Accession: NP_000586

UniProt: P01374

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process