PF4 Antikörper

Produktnummer ABIN116215

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PF4 in WB, IHC (p), EIA und Func. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für PF4 Antikörper (ABIN116215)

Target: PF4 (Platelet Factor 4 (PF4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-PF4 Antikörper

Spezifität: This antibody reacts with Human Platelet Factor 4 (PF-4).

Aufreinigung: Affinity Chromatography employing immobilized Human PF-4 matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant human Platelet Factor-4 (hPF-4)

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhPF?4 (50 ng/mL), a concentration of 1.75-3.0 μg/mL of this antibody is required. ELISA: To detect hPF?4 by direct ELISA (using 100l/well antibody solution) a concentrationof at least 0.5g/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hPF?4. Western Blot: To detect hPF?4 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPF?4 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening.Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Details zu PF4

Target: PF4 (Platelet Factor 4 (PF4))

Andere Bezeichnung: Platelet Factor 4 / PF4

Hintergrund: Synonyms: C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4

Gen-ID: 5196

NCBI Accession: NP_002610

UniProt: P02776