PF4 Antikörper

Produktnummer ABIN116215

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PF4 in WB, IHC (p), EIA und Func. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PF4 Antikörper (ABIN116215)

Target: PF4 (Platelet Factor 4 (PF4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-PF4 Antikörper

Spezifität: This antibody reacts with Human Platelet Factor 4 (PF-4).

Aufreinigung: Affinity Chromatography employing immobilized Human PF-4 matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant human Platelet Factor-4 (hPF-4)

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhPF?4 (50 ng/mL), a concentration of 1.75-3.0 μg/mL of this antibody is required. ELISA: To detect hPF?4 by direct ELISA (using 100l/well antibody solution) a concentrationof at least 0.5g/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hPF?4. Western Blot: To detect hPF?4 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPF?4 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening.Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Details zu PF4

Target: PF4 (Platelet Factor 4 (PF4))

Andere Bezeichnung: Platelet Factor 4 / PF4

Hintergrund: Synonyms: C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4

Gen-ID: 5196

NCBI Accession: NP_002610

UniProt: P02776