CCL20 Antikörper

Produktnummer ABIN116171

Der Kaninchen Polyklonal anti-CCL20 Antikörper wird verwendet zum Nachweis von CCL20 in Proben von Human. Er wurde validiert für WB, EIA und Func.

Kurzübersicht für CCL20 Antikörper (ABIN116171)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCL20 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-CCL20 Antikörper

Spezifität: Human MIP-3 alpha (EXODUS-1, LARC).

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant human Macrophage Inflammatory Protein 3 alpha (hMIP-3 alpha).

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhuman MIP-3 alpha (100 ng/mL), a concentration of 15.0 - 18.0g/mL of this antibody isrequired. ELISA: To detect MIP-3 alpha by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antibody allows thedetection of 0.2 - 0.4 ng/well of recombinant MIP-3 alpha. Western Blot: This antibody can be used at a concentration of 0.1 - 0.2 μg/mL The detectionlimit for recombinant MIP-3 alpha is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry on frozen (use 1 μg/mL) and paraffin embedded tissues.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C for one year. Following reconstitution the antibody can be stored at 2-8 °C for 2 weeks.

Haltbarkeit: 12 months

Details zu CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Andere Bezeichnung: MIP3 alpha / CCL20

Hintergrund: Macrophage Inflammatory Protein 3 alpha is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. It inhibits proliferation of myeloid progenitors in colony formation assays. It may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. It binds to CCR6.Synonyms: Beta chemokine exodus-1, C-C motif chemokine 20, CC chemokine LARC, CCL20, DC marker, Dendritic Cell Marker, Exodus-1, LARC, Liver and activation-regulated chemokine, MIP-3 alpha, MIP3A, Macrophage inflammatory protein 3 alpha, SCYA20, Small-inducible cytokine A20

Gen-ID: 6364

NCBI Accession: NP_001123518

UniProt: P78556

Pathways: Phosphorylierungen bei SARS-CoV-2 Infektion