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CCL4 Antikörper

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CCL4 in WB, IHC (p), EIA und Func. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.
Produktnummer ABIN116167

Kurzübersicht für CCL4 Antikörper (ABIN116167)

Target

Alle CCL4 Antikörper anzeigen
CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reaktivität

  • 67
  • 47
  • 21
  • 11
  • 10
  • 10
  • 2
  • 2
  • 2
  • 1
Human

Wirt

  • 113
  • 12
  • 3
  • 2
  • 1
  • 1
  • 1
Kaninchen

Klonalität

  • 117
  • 15
Polyklonal

Konjugat

  • 73
  • 16
  • 13
  • 5
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser CCL4 Antikörper ist unkonjugiert

Applikation

  • 109
  • 53
  • 46
  • 40
  • 38
  • 13
  • 13
  • 13
  • 8
  • 7
  • 6
  • 3
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
  • Spezifität

    Recognizes Human Macrophage Inflammatory Protein-1 beta (MIP-1b). Other species not tested.

    Aufreinigung

    Affinity Chromatography employing immobilized Human MIP-1beta matrix.

    Immunogen

    Highly pure (>98%) E.coli derived recombinant Human MIP-1 beta (Cat.-No PA107)
  • Applikationshinweise

    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMIP-1 beta (50.00 ng/mL), a concentration of 1.75-3.00 μg/mL of this antibody is required. ELISA: To detect hMIP-1 beta by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hMIP-1 beta. Western Blot: To detect hMIP-1 beta by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMIP-1 beta is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Rekonstitution

    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

    Buffer

    PBS, pH 7.2 without preservatives

    Konservierungsmittel

    Without preservative

    Handhabung

    Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
  • Schlaepfer, Audigé, Joller, Speck: "TLR7/8 triggering exerts opposing effects in acute versus latent HIV infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 5, pp. 2888-95, (2006) (PubMed).

  • Target

    CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

    Andere Bezeichnung

    MIP1 beta / CCL4

    Hintergrund

    MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94 % - 98 % identical and these proteins are all approximately 75 % homologous to murine MIP1 beta. MIP1 beta also shares approximately 70 % amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.Synonyms: CCL-4, G-26 T-lymphocyte-secreted protein, HC21, LAG1, Lymphocyte activation gene 1 protein, MIP-1-beta, MIP1B, Macrophage inflammatory protein 1-beta, PAT744, Protein H400, SCYA4, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2

    Gen-ID

    388372

    NCBI Accession

    NP_001001435

    UniProt

    P13236
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