CCL22 Antikörper

Produktnummer ABIN116160

Dieses Anti-CCL22-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CCL22 in WB und EIA. Geeignet für Human.

Kurzübersicht für CCL22 Antikörper (ABIN116160)

Target: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCL22 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CCL22 Antikörper

Spezifität: Human Macrophage Derived Chemokine (MDC, CCL22)

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hMDC (human Macrophage-Derived Chemokine).

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMDC (100 ng/mL), a concentration of 5-7 μg/mL of this antibody is required. ELISA: To detect hMDC by direct ELISA (using 100l/well antibody solution) a concentrationof at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of of 0.2-0.4 ng/well of recombinant hMDC. Western Blot: To detect hMDC by Western Blot analysis this antibody can be used at aconcentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMDC is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Prior to reconstitution best stored desiccated.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior and following at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Details zu CCL22

Target: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Andere Bezeichnung: MDC / CCL22

Hintergrund: The MDC gene is one of several Cys-Cys (CC) cytokine genes which are clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by MDC displays chemotactic activity for natural killer cells, chronically activated T lymphocytes, monocytes and dendritic cells. It has no chemoattractant activity for eosinophils, neutrophils and resting T lymphocytes and also displays a mild activity for primary activated T lymphocytes. The product of this gene binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology.Synonyms: C-C motif chemokine 22, CC chemokine STCP-1, Macrophage-derived chemokine, SCYA22, Small-inducible cytokine A22, Stimulated T-cell chemotactic protein 1

Gen-ID: 6367

NCBI Accession: NP_002981

UniProt: O00626