CCL7 Antikörper

Produktnummer ABIN116147

Der Ziege Polyklonal Anti-CCL7-Antikörper wurde für WB und EIA validiert. Er ist geeignet, CCL7 in Proben von Human zu detektieren.

Kurzübersicht für CCL7 Antikörper (ABIN116147)

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CCL7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CCL7 Antikörper

Spezifität: This antibody recognizes Human Macrophage Chemotactic Protein-3 (MCP-3/CCL7). Other species not tested.

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hMCP?3 (human Macrophage/Monocyte chemotactic protein-3).

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMCP-3 (100.0 ng/mL), a concentration of 3.0-4.0 μg/mL of this antibody is required. ELISA: (Indirect): To detect hMCP-3 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hMCP-3. (Sandwich): To detect hMCP-3 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human MCP-3 (PP1046B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMCP-3. Western Blot: To detect hMCP-3 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMCP-3 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Centrifuge vial prior to opening!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody in the dark at 2-8 °C. Do Not Freeze! Avoid prolonged exposure to light.

Details zu CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Andere Bezeichnung: MCP3 / CCL7

Hintergrund: This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.Synonyms: C-C motif chemokine 7, CCL-7, MCP-3, Macrophage Chemotactic Protein-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, SCYA6, SCYA7, Small-inducible cytokine A7

Gen-ID: 6354

NCBI Accession: NP_006264

UniProt: P80098