CCL1 Antikörper (Biotin)

Produktnummer ABIN116045

Der Kaninchen Polyklonal Anti-CCL1-Antikörper wurde für WB und EIA validiert. Er ist geeignet, CCL1 in Proben von Human zu detektieren.

Kurzübersicht für CCL1 Antikörper (Biotin) (ABIN116045)

Target: CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCL1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CCL1 Antikörper (Biotin)

Spezifität: Recognizes Human I-309

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant Human I-309. Remarks: Molecular Weight: ~150 kDa

Anwendungsinformationen

Applikationshinweise: Direct ELISA: To detect Human I-309 by Direct ELISA (using 100 μL/well) a concentration ofapprox. 1.0 μg/mL of this antibody is required. This Biotinylated polyclonal antibody allows thedetection of at least 0.2-0.4 ng/well of recombinant Human I-309. Sandwich ELISA: To detect Human I-309 by Sandwich ELISA (using 100 μL/well) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylated polyclonalantibody, in conjunction with anti-Human I-309 (PP1017P1 or PP1017P2) as a Captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human I-309. Western Blot: To detect Human I-309 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human I-309 is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu CCL1

Target: CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

Andere Bezeichnung: CCL1 / I-309

Hintergrund: I-309 is a member of the C-C, or beta chemokine class. Other chemokines in this group include MIP1 alpha, MIP1 beta, RANTES, MCP1/2/3/4, mouse JE, 1 - 2 μg/mL antibody detects 20 and mouse MARC, eotaxin, HCC1 and C10. These chemokines act primarily as chemoattractants and activate monocytes, dendritic cells, T lymphocytes, natural killer cells, B lymphocytes, basophils and eosinophils. I-309 was originally identified by subtractive hybridization as a transcript that was present in a gamma/delta T cell line but not in EBVtransformed B cells. I-309 is a chemoattractant for monocytes, but not for neutrophils. IP-10 is an approximately 8.5 kD polypeptide of 73 amino acids. The precursor form of human I-309 consists of 96 amino acids. To generate the mature I-309, the precursor cleaves its hydrophobic signal peptide. Human I-309 is thought to be a homologue of the mouse TCA3, and shows 42 % amino acid homology to mouse TCA3.Synonyms: C-C motif chemokine 1, CCL-1, SCYA1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309

Gen-ID: 6346

NCBI Accession: NP_002972

UniProt: P22362