CXCL2 Antikörper

Produktnummer ABIN116031

Dieses Anti-CXCL2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CXCL2 in WB, EIA und Func. Geeignet für Human.

Kurzübersicht für CXCL2 Antikörper (ABIN116031)

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCL2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-CXCL2 Antikörper

Spezifität: This antibody detects GRO-beta.

Aufreinigung: Affinity Chromatography employing immobilized hGRO-beta matrix.

Immunogen: Highly pure (>98%) recombinant hGRO-beta (human GRO-beta)

Anwendungsinformationen

Applikationshinweise: Neutralization(To yield one-half maximal inhibition [ND50] of the biological activity of hGRO-beta (100.00 ng/mL), a concentration of 0.5-1.0 μg/mL of this antibody has been used with previous lots)ELISA(Indirect): To detect hGRO-beta by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hGRO-beta. (Sandwich): To detect hGRO-beta by sandwich ELISA (using 100 μL/well antibody solution)a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with biotinylated anti-Human GRO-beta as a detection antibody,allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGRO-beta. Western BlotTo detect hGRO-beta by Western Blot analysis this antibody can be used at a concentrationof 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hGRO-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.

Haltbarkeit: 12 months

Details zu CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Andere Bezeichnung: CXCL2 / MIP2A

Hintergrund: GRO beta is a member of the CXC, or chemokine class. It contains the ELR domain immediately preceding the first cysteine residue near the amino terminus. Other chemokines in this group include IL8, GRO alpha/beta/gamma, mouse KC, ENA78, GCP2, PBP/CTAPIII/beta TG/NAP2. These chemokines act primarily on neutrophils as chemoattractants and activators, including neutrophil degradation with release of myloperoxidase and other enzymes. GRO beta was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. GRO beta is an approximately 8 kDa polypeptide of 73 amino acids. The precursor form of GRO beta consists of 100 amino acids. To generate the mature GRO beta, the precursor cleaves its amino terminal 27 amino acids. GRO beta shows 60 % amino acid homology to human GRO alpha and GRO gamma.Synonyms: C-X-C motif chemokine 2, GRO2, GROB, Gro-beta, Growth-regulated protein beta, MIP-2A, MIP2-alpha, Macrophage inflammatory protein 2-alpha, SCYB2

Gen-ID: 2920

NCBI Accession: NP_002080

UniProt: P19875

Pathways: Cellular Response to Molecule of Bacterial Origin