CXCL5 Antikörper

Produktnummer ABIN115997

Der Kaninchen Polyklonal anti-CXCL5 Antikörper wird verwendet zum Nachweis von CXCL5 in Proben von Human. Er wurde validiert für WB, EIA und Func.

Kurzübersicht für CXCL5 Antikörper (ABIN115997)

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCL5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-CXCL5 Antikörper

Spezifität: Human Epithelial Neutrophil Activity Protein-78 (ENA-78).

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hENA-78.

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhENA-78 (1.0 ng/mL), a concentration of 15-20 μg/mL of this antibody is required. ELISA: To detect hENA-78 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hENA-78. Western Blot: To detect hENA-78 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Centrifuge vial prior to opening!

Lagerung: 4 °C

Details zu CXCL5

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Andere Bezeichnung: CXCL5 / ENA78

Hintergrund: CXCL5 is a a 9.9kD inflammatory chemokine that belongs to the CXC chemokine family. CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils. This chemokine is produced concomitantly with Interleukin 8 (IL8) in response to stimulation with either Interleukin 1 (IL1) or Tumor Necrosis Factor alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.Synonyms: C-X-C motif chemokine 5, Cytokine LIX, ENA-78, Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, SCYB5, Small-inducible cytokine B5

Gen-ID: 6374

NCBI Accession: NP_002985

UniProt: P42830

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity