CXCL5 Antikörper (Biotin)

Produktnummer ABIN115995

Der Biotin-konjugierte Kaninchen Polyklonal anti-CXCL5 Antikörper (ABIN115995) detektiert spezifisch CXCL5 in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CXCL5 Antikörper (Biotin) (ABIN115995)

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCL5 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CXCL5 Antikörper (Biotin)

Spezifität: This antibody reacts with human Epithelial Neutrophil Activity Protein-78 (hENA-78)

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hENA-78 (human ENA-78).

Anwendungsinformationen

Applikationshinweise: ELISA: (Direct): To detect hENA-78 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hENA-78. (Sandwich): To detect hENA-78 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Human ENA-78 (PP1003P) as a captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hENA-78. Western Blot: To detect hENA-78 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu CXCL5

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Andere Bezeichnung: CXCL5 / ENA78

Hintergrund: CXCL5 is a a 9.9kD inflammatory chemokine that belongs to the CXC chemokine family. CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils. This chemokine is produced concomitantly with Interleukin 8 (IL8) in response to stimulation with either Interleukin 1 (IL1) or Tumor Necrosis Factor alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.Synonyms: C-X-C motif chemokine 5, Cytokine LIX, ENA-78, Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, SCYB5, Small-inducible cytokine B5

Gen-ID: 6374

NCBI Accession: NP_002985

UniProt: P42830

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity