CCL5 Antikörper

Produktnummer ABIN115981

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch CCL5 in WB, Func und EIA. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für CCL5 Antikörper (ABIN115981)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCL5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Produktdetails anti-CCL5 Antikörper

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant rRANTES.

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofrRANTES (100 ng/mL), a concentration of 3.0-5.0 μg/mL of this antibody is required. ELISA: To detect rRANTES by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of0.2-0.4 ng/well of recombinant rRANTES. Western Blot: To detect rRANTES by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rRANTES is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu CCL5

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Andere Bezeichnung: RANTES / CCL5

Hintergrund: Synonyms: C-C motif chemokine 5, D17S136E, E °CP, Eosinophil-chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, cell-specific protein P228

Gen-ID: 10116

UniProt: P50231

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration