ELISA: To detect rat RANTES by direct ELISA (using 100 ml/well antibody solution) thisantibody can be used at a concentration of 0.15-0.30 g /mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinantrat RANTES. Western Blot: To detect rat RANTES by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 g /mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rat RANTES is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Konservierungsmittel
Without preservative
Handhabung
Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Target
CCL5
(Chemokine (C-C Motif) Ligand 5 (CCL5))
Andere Bezeichnung
RANTES / CCL5
Hintergrund
Synonyms: C-C motif chemokine 5, D17S136E, E °CP, Eosinophil-chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, cell-specific protein P228