CCL24 Antikörper

Produktnummer ABIN115834

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CCL24 in WB, IHC (p) und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CCL24 Antikörper (ABIN115834)

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CCL24 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-CCL24 Antikörper

Spezifität: This antibody detects Human Eotaxin-2.

Aufreinigung: Antigen Affinity Chromatography

Immunogen: Highly pure (>98%) recombinant Human Eotaxin-2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: In a Sandwich ELISA (assuming 100μL/well), a concentration of 2.0-4.0 μg/mL of thisantibody will detect at least 80.0 pg/well of recombinant Human Eotaxin-2 when used withBiotin anti-Human Eotaxin-2 antibody (cat. AP01125BT) as the detection antibody at aconcentration of approximately 1.0-2.0 μg/mL. Western Blot: To detect Human Eotaxin-2 by Western Blot analysis this antibody can beused at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant Human Eotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Konservierungsmittel: Without preservative

Vorsichtsmaßnahmen: Do Not Add Sodium Azide

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Details zu CCL24

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Andere Bezeichnung: Eotaxin-2 / CCL24

Hintergrund: Eotaxin 2 is a member of the CC chemokine family, based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin 2 cDNA encodes a 119 amino acid residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N glycosylation site. Mature human eotaxin 2 has a predicted molecular mass of approximately 10.6 kDa. Compared to other CC chemokines, eotaxin 2 exhibits 40 %, 42 %, and 39 % amino acid identity to MCP3, MIP1 alpha, and eotaxin, respectively. Human CC chemokine eotaxin 2 maps to chromosome 7q11.23. Both Eotaxin and eotaxin 2 activate and attract eosinophils and basophils. A receptor for human eotaxin has been identified and found to be the third numbered receptor in the C-C chemokine subfamily of receptors (CCR3. On eosinophils, the effects of eotaxin 2 is inhibited by an CCR3 antibody and cross-desensitized by eotaxin and MCP4, suggesting that all three CC chemokines act through CCR3. Eotaxin 2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant eotaxin 2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin 2 inhibits colony formation in myleloid progenitor cells.Synonyms: C-C motif chemokine 24, CK-beta-6, Eosinophil chemotactic protein 2, MPIF-2, MPIF2, Myeloid progenitor inhibitory factor 2, SCYA24, Small-inducible cytokine A24

Gen-ID: 6369

NCBI Accession: NP_002982

UniProt: O00175

Pathways: Regulation of Actin Filament Polymerization