CCL7 Antikörper

Produktnummer ABIN115768

Dieser Maus Monoklonal Antikörper detektiert spezifisch CCL7 in WB, EIA und Func. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CCL7 Antikörper (ABIN115768)

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CCL7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-CCL7 Antikörper

Spezifität: Recognizes Human Macrophage Chemotactic Protein-3 (MCP-3)

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Recombinant Hunan MCP-3.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman MCP-3 (100 ng/mL), a concentration of 1-3 μg/mL of this antibody is required. ELISA: In a Sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL ofthis antibody will detect recombinant Human MCP-3 when used with Biotin conjugatedanti-Human MCP-3 antibody (cat. PP1046B) as the detection antibody at a concentration ofapproximately 0.5-1.0 μg/mL. Western Blot: To detect Human MCP-3 by Western Blot analysis this antibody can be usedat a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human MCP-3 is 2.0-4.0 ng/lane, underreducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Andere Bezeichnung: MCP3 / CCL7

Hintergrund: This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.Synonyms: C-C motif chemokine 7, CCL-7, MCP-3, Macrophage Chemotactic Protein-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, SCYA6, SCYA7, Small-inducible cytokine A7

Gen-ID: 6354

NCBI Accession: NP_006264

UniProt: P80098