LYVE1 Antikörper (AA 24-227)

Produktnummer ABIN115689

Dieses Anti-LYVE1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von LYVE1 in WB, FACS, IHC (p), IF, EIA und IHC (fro). Geeignet für Maus. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für LYVE1 Antikörper (AA 24-227) (ABIN115689)

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LYVE1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-LYVE1 Antikörper

Bindungsspezifität: AA 24-227

Spezifität: Highly specifc for Mouse Lyve-1. No cross reactivities observed so far.

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Aufreinigung: Antigen Affinity Chromatography

Immunogen: Highly pure recombinant Mouse soluble LYVE-1 produced in insect cells (Cat.-No DA3524) Remarks: The recombinant soluble LYVE-1 consists of amino acid 24 (Ala) to 228 (Gly) and is fused to a C-terminal His-tag (6xHis).

Anwendungsinformationen

Applikationshinweise: ELISA (1-15 μg/mL). Western blot (1-2 μg/mL). FACS analysis (3-20 μg/mL). Immunohistochemistry on Paraffin and Frozen Sections (0.25-4 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in distilled sterile Water to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.4, without preservatives or stabilizers

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized IgG is stable at 2-8 °C for one month and at -20 °C for longer. When reconstituted the antibody is stable for at least six weeks at 2-8 °C. For longer store in aliquots at -20 °C.

Referenzen für anti-LYVE1 Antikörper (ABIN115689)

Nakao, Zandi, Faez, Kohno, Hafezi-Moghadam: "Discontinuous LYVE-1 expression in corneal limbal lymphatics: dual function as microvalves and immunological hot spots." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 26, Issue 2, pp. 808-17, (2012) (PubMed).

Heishi, Hosaka, Suzuki, Miyashita, Oike, Takahashi, Nakamura, Arioka, Mitsuda, Takakura, Hojo, Matsumoto, Yamauchi, Ohta, Sonoda, Sato: "Endogenous angiogenesis inhibitor vasohibin1 exhibits broad-spectrum antilymphangiogenic activity and suppresses lymph node metastasis." in: The American journal of pathology, Vol. 176, Issue 4, pp. 1950-8, (2010) (PubMed).

Conrad, Niess, Huss, Huber, von Luettichau, Nelson, Ott, Jauch, Bruns: "Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo." in: Circulation, Vol. 119, Issue 2, pp. 281-9, (2009) (PubMed).

Details zu LYVE1

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Andere Bezeichnung: LYVE-1

Hintergrund: LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41 % similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 Molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 Molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.Synonyms: CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, HAR, Hyaluronic acid receptor, LYVE1, Lymphatic vessel endothelial hyaluronic acid receptor 1, XLKD1

Gen-ID: 114332

NCBI Accession: NP_444477

UniProt: Q8BHC0

Pathways: Glycosaminoglycan Metabolic Process