LYVE1 Antikörper (AA 24-228)

Produktnummer ABIN115688

Der Kaninchen Polyklonal Anti-LYVE1-Antikörper wurde für WB, FACS, IF, EIA und IHC (fro) validiert. Er ist geeignet, LYVE1 in Proben von Maus zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für LYVE1 Antikörper (AA 24-228) (ABIN115688)

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LYVE1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-LYVE1 Antikörper

Bindungsspezifität: AA 24-228

Spezifität: This antibody detetcs Lyve-1.

Kreuzreaktivität (Details): Species reactivity (tested):Mouse. This antibody is not reactive with Human LYVE-1.

Aufreinigung: Protein G Chromatography (+ his tag depletetion)

Immunogen: Highly pure recombinant Mouse soluble LYVE-1 produced in insect cells. This recombinant soluble LYVE-1 consists of amino acid 24 (Ala) to 228 (Gly) and is fused to a C-terminal His-tag (6xHis).

Anwendungsinformationen

Applikationshinweise: ELISA (1-15 μg/mL). Western blot (1-2 μg/mL). FACS analysis (3-20 μg/mL). Immunohistochemistry on Frozen Sections (1-5 μg/mL). For formalin-fixed, paraffin-embedded sections use the immunogen affinity purifiedantibody ABIN115689.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water/PBS to a concentration of > 0.5 mg/mL.

Buffer: PBS, pH 7.2 without preservatives or stabilizers

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized IgG is stable at 2-8 °C for one month and at -20 °C for longer. When reconstituted the antibody is stable for at least six weeks at 2-8 °C. For longer store in aliquots at -20 °C.

Referenzen für anti-LYVE1 Antikörper (ABIN115688)

Rangel-Moreno, de la Luz Garcia-Hernandez, Ramos-Payan, Biear, Hernady, Sangster, Randall, Johnston, Finkelstein, Williams: "Long-Lasting Impact of Neonatal Exposure to Total Body Gamma Radiation on Secondary Lymphoid Organ Structure and Function." in: Radiation research, Vol. 184, Issue 4, pp. 352-66, (2015) (PubMed).

Heishi, Hosaka, Suzuki, Miyashita, Oike, Takahashi, Nakamura, Arioka, Mitsuda, Takakura, Hojo, Matsumoto, Yamauchi, Ohta, Sonoda, Sato: "Endogenous angiogenesis inhibitor vasohibin1 exhibits broad-spectrum antilymphangiogenic activity and suppresses lymph node metastasis." in: The American journal of pathology, Vol. 176, Issue 4, pp. 1950-8, (2010) (PubMed).

Conrad, Niess, Huss, Huber, von Luettichau, Nelson, Ott, Jauch, Bruns: "Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo." in: Circulation, Vol. 119, Issue 2, pp. 281-9, (2009) (PubMed).

Details zu LYVE1

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Andere Bezeichnung: LYVE-1

Hintergrund: LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41 % similar to the CD44 HA receptor with a 212- esidue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 Molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE- molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymphspecific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.Synonyms: CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, HAR, Hyaluronic acid receptor, LYVE1, Lymphatic vessel endothelial hyaluronic acid receptor 1, XLKD1

Gen-ID: 114332

NCBI Accession: NP_444477

UniProt: Q8BHC0

Pathways: Glycosaminoglycan Metabolic Process