LYVE1 Antikörper (AA 24-231)

Produktnummer ABIN115663

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch LYVE1 in WB, FACS, IHC (p), EIA und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für LYVE1 Antikörper (AA 24-231) (ABIN115663)

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LYVE1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-LYVE1 Antikörper

Bindungsspezifität: AA 24-231

Spezifität: This antibody will detect LYVE-1 on the surface of lymphatic endothelial cells by Immunohistochemistry. It detects a 70 kDa LYVE-1 band in Western blotting. This antibody is not reactive with Mouse LYVE-1.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Antigen Affinity Chromatography

Immunogen: Highly pure recombinant Human soluble LYVE-1 produced in insect cells. It consists of amino acid 24 (Ser) to 232 (Gly) and is fused to a C-terminal His-tag (6xHis).

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen and Paraffin Embedded Sections (1-5 μg/mL). Heat mediated antigen retrieval is recommended when staining paraffin embeddedsections. Western blot (1-2 μg/mL). ELISA (1-15 μg/mL). Flow Cytometry (3-20 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL

Buffer: 5 mM PBS, pH 7.2 without preservatives or stabilizers

Konservierungsmittel: Without preservative

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. Protect from Light!

Details zu LYVE1

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Andere Bezeichnung: LYVE-1

Hintergrund: LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosamino-glycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41 % similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 Molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 Molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels.Synonyms: CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, HAR, Hyaluronic acid receptor, LYVE1, Lymphatic vessel endothelial hyaluronic acid receptor 1, XLKD1

Gen-ID: 10894

NCBI Accession: NP_006682

UniProt: Q9Y5Y7

Pathways: Glycosaminoglycan Metabolic Process