Ezrin Antikörper (AA 362-585)

Produktnummer ABIN115201

Dieser Maus Monoklonal Antikörper detektiert spezifisch Ezrin in WB, IHC (p) und IP. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh), Hamster und Känguruh. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für Ezrin Antikörper (AA 362-585) (ABIN115201)

Target: Ezrin (EZR)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hamster, Känguruh

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Ezrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Klon: 3C12

Produktdetails anti-Ezrin Antikörper

Bindungsspezifität: AA 362-585

Spezifität: This antibody reacts with a protein of 81 kDa.
Cellular Localization: Cell membrane

Aufreinigung: Purified

Immunogen: BALB/C mice were immunized with a recombinant fragment of human ezrin corresponding to amino acid 362-585.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation. Western blot: 1: 100-750. Immunohistochemistry on paraffin embedded sections: dilute 1: 50-1: 100 in an ABC method(30 minutes at room temperature). Recommended positive control: Lung.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-Ezrin Antikörper (ABIN115201)

Hatano, Akiyama, Tamura, Hosogi, Marunaka, Caplan, Ueno, Tsukita, Asano: "Knockdown of ezrin causes intrahepatic cholestasis by the dysregulation of bile fluidity in the bile duct epithelium in mice." in: Hepatology (Baltimore, Md.), Vol. 61, Issue 5, pp. 1660-71, (2015) (PubMed).

Fonseca, Freund, Bornhäuser, Corbeil: "Polarization and migration of hematopoietic stem and progenitor cells rely on the RhoA/ROCK I pathway and an active reorganization of the microtubule network." in: The Journal of biological chemistry, Vol. 285, Issue 41, pp. 31661-71, (2010) (PubMed).

Details zu Ezrin

Target: Ezrin (EZR)

Andere Bezeichnung: Ezrin

Hintergrund: Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. Ezrin is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.Synonyms: Cytovillin, EZR, VIL2, Villin-2, p81

Gen-ID: 7430

NCBI Accession: NP_001104547

UniProt: P15311

Pathways: Maintenance of Protein Location