KRT15 Antikörper (C-Term)

Produktnummer ABIN114888

Dieses Anti-KRT15-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von KRT15 in IF und IHC (p). Geeignet für Human, Ratte, Maus und Rind (Kuh).

Kurzübersicht für KRT15 Antikörper (C-Term) (ABIN114888)

Target: KRT15 (Keratin 15 (KRT15))

Reaktivität: Human, Ratte, Maus, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT15 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: LHK15

Produktdetails anti-KRT15 Antikörper

Bindungsspezifität: C-Term

Spezifität: Cytokeratin 15 is a type I cytokeratin without a defined type II partner.
Cellular Localization: Cytoplasmic.

Aufreinigung: Purified

Immunogen: A 17-mer synthetic peptide from the C-terminus of human cytokeratin 15

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunoflourescence. Immunohistochemistry on Paraffin Embedded Sections: 1/25-1/75 dilution in an ABCmethod (30 minutes at room temperature). Requires high temperature antigen unmaskingwith 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu KRT15

Target: KRT15 (Keratin 15 (KRT15))

Andere Bezeichnung: Cytokeratin 15

Hintergrund: Cytokeratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. A number of human cytokeratins are expressed during the development of stratified epithelia from one-layered polar epithelia and continue to be expressed in several adult epithelial tissues. Cytokeratin 15 (CK15) is a type I keratin without a defined type II partner whose expression in epidermal diseases has not been investigated. A monoclonal antibody raised against the last 17 amino acids of the CK15 polypeptide show that CK-15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Although CK-15 in normal hair follicles was virtually absent from hair bulbs, it was expressed by a subset of keratinocytes in the outer root sheath. In human conjunctival epithelium, strong expression of CK-15 was observed in basal cells, whereas CK19 was expressed in both basal and suprabasal layers. Although the expression of K15 and K19 differ in humans and mice, specific staining patterns can be used to characterize the epithelial phenotype in normal and diseased ocular surface.Synonyms: CK-15, Cytokeratin-15, K15, KRT15, KRTB, Keratin, Keratin 15, Keratin-15, Type I Cytoskeletal 15

Gen-ID: 3866

NCBI Accession: NP_002266

UniProt: P19012