Cytokeratin 19 Antikörper

Produktnummer ABIN114797

Der Maus Monoklonal anti-Cytokeratin 19 Antikörper wird verwendet zum Nachweis von Cytokeratin 19 in Proben von Human. Er wurde validiert für WB, IF und IHC (p).

Kurzübersicht für Cytokeratin 19 Antikörper (ABIN114797)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 19 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: Ks19-1 (A53-B-A2)

Produktdetails anti-Cytokeratin 19 Antikörper

Spezifität: Specific for the rod domain (aa 312-335) of Human Cytokeratin 19. In Western blotting 40 kDa and 19 kDa bands are observed.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Human breast cancer cell line MCF-7

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot. Immunofluorescence. Immunohistochemistry on Paraffin Sections: 1/100-1/200 (Requires protein digestionpre-treatment of paraffin sections e. g. trypsin or pronase). Use appendix as Positive Control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Andere Bezeichnung: Cytokeratin 19

Hintergrund: This protein is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumours. Keratin 19 is often co-expressed with keratin 7.Synonyms: CK19, Cytokeratin-19, K19, KRT19, Keratin type I cytoskeletal 19, Keratin-19

Gen-ID: 3880

NCBI Accession: NP_002267

UniProt: P08727