Reactive with natural and recombinant Human MCP-3. Does not show any cross reaction with other Human Cytokines or growth factors tested such as IL1 beta, IL-8, MCAF, SAA and EGF.
CCL7
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
RB36660
unconjugated
Applikationshinweise
ELISA: CCL7 antibody can be used as a Capture antibody in Sandwich ELISA applicationsfor Human MCP-3 Detection in combination with a monoclonal tracer/detection antibody. Suggested Capture Coating Dose: 3 μg/well. Substrate: TMB. Western Blot: An antibody concentration of 0.5-2 μg/mL will allow visualization of 0.1 μg/lane of Human MCP-3. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with Double distillated to adjust the final concentration to 1.00 mg/mL
Buffer
0.01 M PBS, pH 7.2 without preservatives
Konservierungsmittel
Without preservative
Handhabung
This product is photosensitive and should be protected from light
Macrophage/monocyte chemotactic protein-3 (MCP-3), like other two closely related chemokines (MCP1 and MCP-2), is chemotactic to monocytes, but not to neutrophils. MCP-3 showed 78 % sequence homology with MCP-1 and 58 % sequence homology with MCP-2. The primary receptor for MCP-3 is D6. MCP-3 also competes with MCP-1 to bind to CCR10, and competes with Rantes and MIP-1α to bind to CCR1. MCP-3 stimulates macrophage to secrete proteases. A variety of cancer cell lines express MCP-3, suggesting a role in tumor invasion and metastasia.Synonyms: C-C motif chemokine 7, CCL-7, MCP-3, Macrophage Chemotactic Protein-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, SCYA6, SCYA7, Small-inducible cytokine A7